service - Negative staining B A C K
Get a new perspective on sample quality with visual feedback
Negative staining is a necessary procedure to evaluate the behavior of biomolecules. After purification, the protein will be stained with heavy metal and checked under a low-end electron microscope, thus having a very quick look to check if the protein is in good homogeneity at a low cost.
Negative staining Workflow
Why do you need Negative staining Services?
● Catch a glimpse of sample purity and homogeneity
● Check if the purification conditions suitable for specimen preparation
● Detection of 2D characterization of biomolecules
About the samples
SDS-PAGE to check no polluted or degraded protein bands. Make sure it is the target protein (mass spectrometry identification, etc.)
Do not contain organic substances such as polysaccharides, DMSO, glycerol. Salt concentration should below 300mM.
Samples should be divided into aliquots, flash frozen and stored in liquid nitrogen. Avoid repeated freezing and thawing.